Healthcare Professional
HESA-A is a natural biologic compound of herbal and marine origin (Wild celery, Cumin and King Prawn) with anti-cancer, anti-inflammatory, immuno-modulatory, anti-oxidant, and metabolic effects. It is composed of organic and mineral constituents. HESA-A also contains traces of vanadium(V), nickel(Ni), titanium(Ti), zinc (Zn), Strontium (Sr), and selenium(Se) that are known for their anti-oxidant properties. Although its mechanism of action has yet to be fully elucidated, HESA-A’s activity may be attributed to the anti-oxidant properties of the elements contained in the drug complex.
HESA-A is Selective Towards Cancer Cells:
The cytotoxicity of HESA-A was evaluated using four cancer (MDA-MB-468 breast, Hep2, HeLa) and two normal (L929 and McCoy) cell lines.
The stock solution (0.8 mg/ml, pH 7.4) of HESA-A was sterilized using 0.22 μ microbiological filters and diluted to final concentrations of 0.4, 0.2, 0.1 and 0.05 mg/ml.
180 μl of cells were grown in complete RPMI1640 and seeded in 96 well micro plates at a concentration of 1-5 x 104 cells/ml. After incubation for 24 hours, 20 μl of different concentrations of HESA-A was added and cells were incubated further for 72 hours. Using MTT assay, percent cell survival was determined by ELISA at 540 nm. Doxorubicin was used for comparison.
Dose response curve for MDA-MB 468, Hela, and HepII cells following 96 hours continuous exposure to HESA-A, n >= 5
HESA-A (0.1 mg/ml) reduced the number of viable MDA-MB-468 cells to less than 50%.
For HeLa and Hep2 cells the IC50's were 0.2 and 0.4 mg/ml, respectively. In normal cells IC50 was not obtained at any given concentration. These results suggest that HESA-A selectively inhibits the growth of cancer cells in a dose dependent manner.
Dose response curve for McCoy and L929 cells following 96 hours continuous exposure to HESA-A, n >=8
Conclusion: HESA-A demonstrated anti-tumor effects in both in vitro and in vivo studies. HESA-A inhibited the growth of cancer cells in a selective and dose-dependent manner. Normal cells were unaffected by the compound even at the highest dose tested (5.4 mg/ml).